Fig. 2.

Humoral IgY responses in chicken after the 4th immunization analyzed by ELISA. Purified α-enolase protein and bovine serum antigen (BSA) were coated on plate wells. A series of diluted IgY antibodies were examined for their specific binding activity to either α-enolase or BSA. Solid and open bars represent the binding of IgY from 4th- or pre-immunized chickens to α-enolase, respectively. In addition, their binding to BSA presented by deep and light gray bars were shown in parallel as negative controls.