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PCR amplification of the variable regions in chicken immunoglobulin genes. Variable regions of the light chain (EnVL) and heavy chain with short-linker (EnVH.S) or with long-linker (EnVH.L) were amplified successfully (panel A). The second round of PCR generated the full-length scFv gene fragments with short (EnscFv.S) or long (EnscFv.L) linkers (panel B).
