Fig. 5.

Sequence analysis of VL and VH genes of scFv antibodies. The nucleotide sequences of VH and VL of 10 clones were determined and translated into amino acid sequences to be aligned with those of the chicken germline gene. FR: framework region; CDR: complementarity-determining region. Sequence gaps were introduced to maximize the alignment; these are indicated by blank spaces. Dots indicate the consensus sequences. Framework region (FR) and complementarity-determining region (CDR) boundaries are indicated above germline sequences.