Table 2.
Validation of microarray data with quantitative real time-PCR (qRT-PCR)
| Experimentsa | Differentially expressed genes |
|
|---|---|---|
| Microarrayb | Validated/selectedc (qRT-PCR) | |
| Effect of surgery | 180 (p < 0.05) | 17/25 |
| Bovine rotavirus | 1203 (p < 0.2) | 42/48 |
| Bovine coronavirus | 1205 (p < 0.2) | 18/26 |
a
Intestinal “loop” experiments with gene expression compared within the same animal.
b
The number of differentially expressed genes identified following microarray analyses (1.5-fold change) is indicated with the significance level in brackets.
c
The numerator represents the number of genes validated by qRT-PCR following selection of gene targets by microarray analysis (denominator).