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. Author manuscript; available in PMC: 2020 Aug 17.
Published in final edited form as: Nat Immunol. 2020 Feb 17;21(3):331–342. doi: 10.1038/s41590-020-0598-4

Fig. 2: GCBCs rely on FAO.

Fig. 2:

a, Measurement of basal OCR values of naive, in vivo–activated or GCBCs, prepared as in Extended Data Fig. 1 and treated with 40uM etomoxir or its vehicle prior to analysis. Raw OCR values (left) from multiple experiments and proportion of total OCR that is etomoxir-sensitive (right; calculated as: [etomoxir inhibited OCR – rotenone/AA OCR] / [basal OCR – rotenone/AA OCR]). b, Representative OCR trace of in vivo activated (left) or GCBCs pre-treated with etomoxir or its vehicle control (center). Symbols are means of three replicate wells and error bars are +/− SEM. Traces marked “palm+BSA” were from wells with added palmitate-conjugated BSA, used to identify the contribution of exogenous fatty acids to etomoxir-sensitive oxygen consumption. Right: Tabulated data that indicate percent contribution of palmitate to etomoxir-sensitive OCR (calculated as: OCRpalm - OCRBSA / OCRpalm - OCReto). c, Percent of FFA-mediated OCR that was stimulated by palmitate addition to in vivo activated or early (d8), peak (d13) and late (d23) GCBCs (calculated as in right of (b)). Bars represent mean +/− SEM; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001 by unpaired, two-tailed t-test.