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. 2020 Apr 1;6(14):eaaz4344. doi: 10.1126/sciadv.aaz4344

Fig. 1. MAP6 localizes inside microtubules.

Fig. 1

(A) Cryo–electron microscopy images showing the two types of microtubules extracted from primary cultured neurons of mouse embryos. Black and white stars indicate microtubules with and without intraluminal particles, respectively. Inset shows a high-magnification image of the area denoted by the dashed rectangle (a band-pass filter was applied and added to the original image to improve contrast). The percentages of microtubules with or without these particles in wild-type (WT) versus MAP6-KO neurons are indicated on the right. The total length of measured microtubules was 278 and 242 μm for wild-type and MAP6-KO conditions, respectively. Scale bars, 50 nm (horizontal) and 25 nm (vertical). (B) Main panels: Cryo–electron tomography of microtubules copolymerized in vitro with purified tubulin and MAP6 in the presence of GMPCPP. Arrowheads, microtubule inner particles. Bottom: (a to d) Transverse sections along the lines indicated in top panels showing localization of dots inside hollow tubes (a and b) or at the inner side of open protofilament sheets (c and d). (C) Cryo–electron microscopy images of in vitro pre-polymerized and taxol-stabilized microtubules (MTs), incubated with or without MAP6. Scale bars, 50 nm.