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. 2020 Apr 1;6(14):eaaz4344. doi: 10.1126/sciadv.aaz4344

Fig. 3. MAP6Δ4-35 mutant stabilizes microtubules but does not lead to catastrophes, curvature, apertures, or particle densities within the lumen.

Fig. 3

(A) Scheme of MAP6 and MAP6Δ4-35 showing the temperature-sensitive (Mc) and temperature-insensitive (Mn) microtubule stabilization domains and the deletion present in the MAP6Δ4-35 mutant. (B) TIRF images of microtubules grown for 90 min from GMPCPP seeds in the presence of either 200 nM MAP6-GFP or MAP6Δ4-35-GFP. Arrowheads point to the dashed appearance of microtubules. Scale bar, 5 μm. (C) Dynamical parameters of microtubules polymerized in the absence or presence of either MAP6-GFP or MAP6Δ4-35-GFP (n = 93, 29, and 43 microtubules, respectively). (D) Electron microscopy images of microtubules copolymerized with either MAP6-GFP or MAP6Δ4-35-GFP in the presence of GMPCPP and processed for negative staining. Scale bar, 50 nm. (E) Aperture frequency in microtubules copolymerized in the absence or presence of either MAP6-GFP or MAP6Δ4-35-GFP. *P < 0.05; ***P < 0.001, Kruskal-Wallis ANOVA followed by post hoc Dunn’s multiple comparison. ns, not significant.