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Epitopes mapped with specific single-chain variable fragment (scFv) antibodies using western blot analysis. Panel (A) shows 10 truncated S fragments on a Coomassie blue-stained polyacrylamide gel. Both Ssc35 (B) and Lsc18 (C) scFv antibodies but not cellular lysate (not shown) recognized a truncated S6 fragment spanning amino acid residues 750–1000.
