Fig. 2.

Amplification plots for IBV-positive (purple) and IBV-negative (blue) samples with IPC. Plot coordinates are cycle number vs. the normalized reporter signal, calculated as the fluorescence signal from the reporter dye normalized to the fluorescence signal of the passive reference included in the reaction mix. Blue horizontal line marks the automatically calculated threshold value. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)