Table 3.

Virus detection in tracheal swabs from experimentally infected chickens.

	Direct analysisa		Allantoic fluid PIb		VI
	RT-PCRc	RRT-PCR	RT-PCR	RRT-PCR
Variant 1	6/12	9/12	6/12	10/12	11/12
IS/885/00	0/12	9/12	3/12	12/12	9/12
IS/1201/04	4/12	6/12	9/12	10/12	12/12
Non-infected	0/12	3/12	1/10	3/12	3/12

^aRNA extraction from tracheal swabs sampled five days post-inoculation (PI).

^bRNA extraction from allantoic fluid harvested 48 h PI.

^cRT-PCR targeting the S1 gene.