Fig. 1.

Establishment of epithelial cell cultures from fetal bovine jejunum and ileum. Epithelial cell suspensions obtained from an 180-day-old fetus were cultured in EGF media as described in Section 2. Primary cultures contained foci of both homogeneous and heterogeneous populations of epithelial and fibroblast-like cells. Clusters of cells with ‘cobblestone’ morphology characteristic of epithelial cells were observed in jejunal (A) and ileal (E) primary cultures (P-0). Clusters of spindle-shaped fibroblast-like cells were also present in both the jejunal (B) and ileal (F) primary cultures. An intermingling of epithelial and fibroblasts cells was occasionally observed in both the jejunal (C) and ileal (G) primary cultures. Primary cultures were trypsinized and transferred to new 75 cm² flasks. In contrast to P-0 cultures, upon further passage secondary cultures (P-1) displayed an homogeneous epithelial cell-like morphology with few fibroblasts visible in either the jejunal (D) or ileal (H) cultures. Magnification 200×.