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. 2008 Nov 5;154(1):20–26. doi: 10.1016/j.jviromet.2008.09.019

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Copyright © 2008 Elsevier B.V. All rights reserved.

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Fig. 1 — Western blot analysis of anti-EDIII MAbs with denatured antigen. (A) Reactivity of four MAbs with recombinant EDIII protein, using irrelevant MAb against influenza virus (H5) as a negative control. (B) Reactivity of MAb 2F5 with E proteins from WNV and JEV. Lanes 1–3: lysates of 293T cells transfected with pcDNA4, pcDNA4-WNV E and pcDNA4-JEV E plasmids, respectively; lane 4: inactivated WNV; lane 5: inactivated JEV; lane 6: cell culture supernatant of BHK-21 cells; lanes 7 and 8: the same as lanes 3 and 1, respectively. M: protein molecular weight markers. Left panel (lanes 1–6) was detected with MAb 2F5 and right panel (lanes 7 and 8) was detected with anti-His antibody.