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Sensitivity of AC-ELISA using MAbs. (A) Quantitative analysis using recombinant EDIII protein. 10 ng/0.1 ml was the detection limit. (B) Quantitative analysis using WNV cell culture supernatant. 80-fold dilution of cell culture supernatant was the detection limit, which was 3.95 TCID50/0.1 ml. The broken line indicates the absorbance ratio cutoff value, which was set at 2.
