Table 1.

Comparison between our RT-PCR assay and the RealArt HPA coronavirus RT-PCR (Artus GmbH)

Dilution	RT-PCR with CORO1 and CORO2	RealArt HPA coronavirus RT-PCR (genome copies)
10−3	+	33000
10−4	+	2480
10−5	+	89
10−6	+	19
10−7	+	3
10−8	−	–

Column two shows the result (scored as positive or negative) obtained with our assay using 10 μl of the indicated RNA dilution (see Section 2). For each dilution the assay was done on two different aliquots and the results were in agreement. Column three shows the average (on two experiments) of the measured number of genome copies in 5 μl of the RNA dilution with RealArt HPA coronavirus RT-PCR.