Table 1.

Primers used for cloning of the coding region of HBsAg and HBcAg and RT-PCR

Amplicon	Primer	Polarity	Nucleotide sequence	Position of the 5′-base
HBV S gene	S6C	Sense	5′-gca cac gga att ccg agg act ggg gac cct g-3′	129a
	S7D	Antisense	5′-gac acc aag ctt ggt tag ggt tta aat gta tac c-3′	842a
HBV C gene	HcNCO	Sense	5′-acc atg gac att gac cct tat aaa g-3′	1901a
	Hc-149s	Antisense	5′-agg atc caa caa cag tag ttt ccg g-3′	2348a
	HBc-EV1	Sense	5′-gga tat cat gga cat cga ccc tta t-3′	1901a
	HBc-EV2	Antisense	5′-act cga gac att gag att ccc gag a-3′	2451a
HBV preC/C	HpreC-EV1	Sense	5′-gga tat cat gca act ttt tca cct ctg-3′	1814a
	HBc-EV2	Antisense	5′-act cga gac att gag att ccc gag a-3′	2451a
STAT1	STAT1-1	Sense	5′-cca tgg aaa tca gac agt acc tgg c-3′	431b
	STAT1-3	Antisense	5′-cct tca cat ttc tga ctt tac tgt c-3′	802b
ISG15	ISG15k	Sense	5′-gac ctg acg gtg aag atg ctg g-3′	84c
	ISG15k-2	Antisense	5′-ttc ctc acc agg atg ctc aga gg-3′	337c
β-Actin	A-1	Sense	5′-tgg aat cct gtg gca tcc atg aaa c-3′	879d
	A-2	Antisense	5′-taa aac gca gct cag taa cag tcc g-3′	1224d

The italicized parts of primers S6C, S7D, HcNCO, Hc-149s, HBc-EV1, HpreC-EV1, and HBc-EV2 indicate the specific cleavage sites of restriction enzymes EcoRI, HindIII, NcoI, BamHI, EcoRV, and XhoI, respectively.

^aNumbering of the sequence is according to U95551.

^bNumbering of the sequence is according to NM_007315.2.

^cNumbering of the sequence is according to XM_010527.

^dNumbering of the sequence is according to NM_001101.2.