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. 2012 Nov 19;187(2):413–420. doi: 10.1016/j.jviromet.2012.11.009

Fig. 1.

Fig. 1

(A) Soluble protein fraction of recombinant HCoV-OC43 NP from IPTG-induced cells prepared using different concentrations of CHAPS in 50 mM Tris–HCl (pH 7.3) and 150 mM NaCl buffer. (B) Expression and purification of OC43 NP. Extract of un-induced cells (lane 1), extract of induced cells (lane 2), protein eluted with 15 mM imidazole (lane 3), and purified NP (lane 4).