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(A) Conformational analysis of HCoV-OC43 NP by the VL3-BA predictor of the PONDR program. (B) SDS-PAGE analysis of three truncated fragments of HCoV-OC43 NP, aa 1–173, aa 174–300, and aa 301–448, purified by Ni-NTA chromatography. Antigenicity analysis of three truncated fragments of HCoV-OC43 NP, aa 1–173, aa 174–300, and aa 301–448, against the rabbit polyclonal antibody at dilutions of (C) 1:50,000 and (D) 1:450,000.
