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. 2018 Jun 11;200:26–32. doi: 10.1016/j.imlet.2018.06.003

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© 2018 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.

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Fig. 4 — CD46 co-stimulation expands IFN-γ-producing CD8⁺ T cells. Isolated CD8⁺ and CD4⁺ T cells were stimulated with either αCD3/αCD28 or αCD3/αCD46 for different time-periods as indicated, and analyzed by flow cytometry for the frequency of IFN-γ and IL-10-producing cells using the IL-10 (PE) and IFN-γ (APC) Secretion Assay Detection Kit. Analyses applied gating for live cells. (A) Contour plot of a representative donor (n = 8 donors). NA = no antibodies. (B) Average frequency (n = 8 donors) of IFN-γ⁺ IL-10⁻, IFN-γ⁺IL-10⁺ and IFN-γ⁻IL-10⁺-secreting cells from CD8⁺ (left panel) and CD4⁺ T cells (right panel). Frequency is calculated per live cells. SD for each of the subsets is indicated on top of the bars. Data were analyzed using Wilcoxon signed rank test, * p < 0.05, ** p < 0.01.