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Comparison of the detection rates of PToV by real -time RT-PCR, conventional RT-PCR, and nested PCR assay.
| RT-PCRa |
Nested PCRb |
|||||||
|---|---|---|---|---|---|---|---|---|
| + | − | Total | + | − | Total | |||
| Real-time RT-PCR | + | 7 | 24 | 31 | 17 | 14 | 31 | |
| − | 0 | 55 | 55 | 0 | 55 | 55 | ||
| Total | 7 | 79 | 86 | 17 | 69 | 86 | ||
Percent observed agreement (Po) = (7+55)/86 = 72.1%. Sensitivity = 7/(7+0) = 100%. Specificity = 55/(24+55) = 69.6%. Kappa = 0.272.
Po = (17+55)/86 = 83.7%. Sensitivity = 17/(17+0) = 100%. Specificity = 55/(14+55) = 79.7%. Kappa = 0.608.
