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. 2009 Nov 14;163(2):452–458. doi: 10.1016/j.jviromet.2009.11.012

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Copyright © 2009 Elsevier B.V. All rights reserved.

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Fig. 2 — Standard curve of real-time RT-PCR (RRT-PCR) assay for turkey coronavirus (TCoV). The X-axis shows the concentration of serially diluted RNA in vitro transcribed from standard pTriEx3-6F/6R in log 10 value and the Y-axis indicates the corresponding threshold cycle (Ct) value. The linear quantitative correlation was established from 10² to 10¹⁰ copies/μl with 99.8% coefficient. The detection limit is as low as 10² copies/μl.