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Dynamic range, sensitivity and PCR efficiency. Mean Ct values vs. concentration of (A) serially diluted DNA standard; (B) serially diluted DNA standard spiked into CRCoV negative cDNA; (C) serially diluted cDNA from a CRCoV positive tracheal sample; and (D) TCID50 of serially diluted cell culture grown virus vs. mean copy number/ml determined by the PCR in log scale. Error bars show ±1 S.D.
