Fig. 2.

Insert preparation, expression and confirmation of N (N1/N2/N3) protein in mammalian cells (ATCC 293T). (a) PCR products of N1 (1269 bp), N2 (327 bp) and N3 (942 bp), M: DNA marker. (b) Eukaryotic expression vector (pVAX-1) cloning (BamH1 and Pst1 digestion). (c) Expression of the constructs, determined by western blot analysis with antisera reactive with SARS CoV N was evaluated after transfection of the indicated plasmid expression vectors in ATCC 293T cells. C: control (whole cell lysate transfected with vector), lane 1: vector inserted gene (pVAX-N1/pVAX-N2/pVAX-N3), lane 2: Recombinant SARS N (N1/N3) protein as positive control.