Table 1.
Name | Polarity | Sequence 5′–3′ |
---|---|---|
Aa | Sense | GGATGTTCGCTGGAACAACTATACCAAACCAATTCAAAATGAAGAGCTAACATCTTTAATTAGAGGAGCACAAACAGCAATGGAT |
Ba | Antisense | GCCGCTTGTGTCTCTAAGTCTTTGCAACTTTTTGGCGAGACTATCAACTTCCGATTCCCAGTCCATTTCTTCTTCTTCGGTTTGATGCATTGCTGTTTGTGCT |
Ca | Antisense | TAGAACTTGGTCTTGACTCTGAGGATTGCTTGCCGCTTGTGTCTCTAAG |
S1a | Sense | CGACTGGATGTTCGCTGGAACAA |
AS1a | Antisense | CAGGATAGAACTTGGTCTTGACTC |
R2forb | Sense | ACTAACATCTTTAATTAGAGGAG |
R2revb | Antisense | CTCTAAGTCTTTGCAACTTT |
R2Pbb | Antisense | FAM-TGGCGAGACTATCAACTTCCGAT-TAMRA |
Primers used for assembly and amplification steps of standard template.
Primers used in real-time RT-PCR.