Table 3.
Analysis of samples collected at necroscopy from CPV-2-positive dogs by real-time assays for DNA and mRNA
| Dog | Tissue sample | DNAa | mRNAb | Viral characterization |
|---|---|---|---|---|
| 242/05-A | Brain | 1.86 × 105 | 6.24 × 102 | CPV-2c |
| Heart | 4.21 × 106 | 4.96 × 102 | ||
| Lungs | 1.99 × 106 | Neg | ||
| Liver | 2.41 × 107 | Neg | ||
| Spleen | 2.61 × 107 | Neg | ||
| Kidney | 7.97 × 105 | Neg | ||
| Mesenteric lymphnode | 7.30 × 107 | Neg | ||
| Bladder | 7.93 × 104 | Neg | ||
| 280/05-A | Brain | 1.11 × 106 | 2.82 × 103 | CPV-2a |
| Lungs | 1.68 × 105 | 1.15 × 103 | ||
| Liver | 4.58 × 106 | 3.49 × 103 | ||
| Spleen | 9.14 × 106 | 6.18 × 103 | ||
| Mesenteric lymphnode | 7.27 × 106 | 3.18 × 103 | ||
| 315/06-D | Brain | 3.37 × 103 | Neg | CPV-2b |
| Lungs | Neg | Neg | ||
| Liver | 1.93 × 103 | Neg | ||
| Spleen | Neg | Neg | ||
| 436/06-A | Brain | 2.46 × 108 | 1.22 × 103 | CPV-2a |
| Cerebellum | 1.38 × 108 | 3.24 × 105 | ||
| Thymus | 1.45 × 107 | 4.83 × 103 | ||
| Spleen | 7.06 × 106 | 9.14 × 102 | ||
| Heart | 1.06 × 107 | 8.41 × 102 | ||
| Lungs | 1.86 × 08 | Neg | ||
| Liver | 7.46 × 108 | 1.07 × 103 | ||
| Kidney | 1.18 × 108 | 3.76 × 102 | ||
| Mesenteric lymphnode | 1.78 × 107 | 1.23 × 103 | ||
| 446/06 | Brain | 2.54 × 102 | Neg | CPV-2a |
| Cerebellum | 1.72 × 103 | 3.43 × 102 | ||
| 1/07 | Brain | 1.16 × 102 | Neg | CPV-2a |
| Cerebellum | 4.25 × 104 | 1.41 × 103 | ||
| 12/07-A | Brain | 5.36 × 105 | 2.63 × 104 | CPV-2a |
| Cerebellum | 2.92 × 103 | 2.18 × 106 | ||
| 12/07-B | Brain | 7.18 × 105 | 1.43 × 104 | CPV-2a |
| Cerebellum | 6.86 × 105 | 2.16 × 105 | ||
| 91/07-B | Brain | 3.26 × 107 | 6.28 × 103 | CPV-2a |
| Cerebellum | 1.08 × 107 | 5.55 × 103 | ||
a
Real-time titre are expressed as number of CPV-2 DNA copies/10 μl of template.
b
Real-time titre are expressed as number of CPV-2 mRNA copies/μl of template.