Table 2.
Details of PCR and LAMP primers designed for detection of capsid protein coding sequences of PCV2
| Primer namea | Genome position | Squence |
|---|---|---|
| F | 596–617 | 5′-ATGGGCTGCTAATTTTGCAGAC-3′ |
| B | 961–982 | 5′-TCAATAGGAAATTCAGGGCATG-3′ |
| FIP | 762–783 | 5′-GTACAGTTCCACCTTTAGTCTC+TTTT+ |
| 659–680 | GGTTACCATGGTGAAGAAGTGG-3′ | |
| BIP | 840–861 | 5′-CAACTGCTGTCCCAGCTGTAGA+TTTT+ |
| 910–931 | TCCTCCGTGGATTGTTCTGTAG-3′ | |
a
The primers of F, B, FIP and BIP were for LAMP and each inner primer of LAMP has two binding regions connected by a TTTT spacer. Primers of F and B were also applied in PCR.