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. 2012 Aug 27;186(1):43–48. doi: 10.1016/j.jviromet.2012.08.007

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Copyright © 2012 Elsevier B.V. Published by Elsevier B.V. All rights reserved.

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Fig. 2 — The sensitivities of RT-LAMP, RT-PCR and real-time RT-PCR assays. A serial 10-fold dilution of BVDV RNA derived from CV24 reference strain was used in the RT-LAMP, RT-PCR and real-time RT-PCR. (A) Results of the RT-LAMP products were viewed under ultraviolet (UV) light. (B) Results of the real-time RT-PCR analysis. (C) Results of the RT-PCR analysis. Lane M: 2000-bp DNA marker; Lane N: negative control; Lane 1: 4.67 × 10⁸ copies/tube; Lane 2: 4.67 × 10⁷ copies/tube; Lane 3: 4.67 × 10⁶ copies/tube; Lane 4: 4.67 × 10⁵ copies/tube; Lane 5: 4.67 × 10⁴ copies/tube; Lane 6: 4.67 × 10³ copies/tube; Lane 7: 4.67 × 10² copies/tube; Lane 8: 4.67 × 10¹ copies/tube; Lane 9: 4.67 × 10⁰ copies/tube; Lane 10: 4.67 × 10⁻¹ copies/tube. All experiments were repeated three times and similar results were obtained.