Table 1.
Virus and samples used in the RT-LAMP assay.
| Name | Source | RT-LAMP result |
|
|---|---|---|---|
| Agarose gel electrophoresis | Color change after adding dye | ||
| BVDV1 | |||
| Oregon CV24 | CVCC, China | + | + |
| NADL | CVCC, China | + | + |
| AV68 | CVCC, China | + | + |
| GX-BVDV1 | GVRI | + | + |
| GX-BVDV2 | GVRI | + | + |
| GX-BVDV3 | GVRI | + | + |
| GX-BVDV4 | GVRI | + | + |
| GX-BVDV5 | GVRI | + | + |
| GX-BVDV6 | GVRI | + | + |
| GX-BVDV7 | GVRI | + | + |
| GX-BVDV8 | GVRI | + | + |
| GX-BVDV9 | GVRI | + | + |
| GX-BVDV10 | GVRI | + | + |
| GX-BVDV11 | GVRI | + | + |
| GX-BVDV12 | GVRI | + | + |
| GX-BVDV13 | GVRI | + | + |
| BVDV2 | |||
| GX-041 | GVRI | + | + |
| Other bovine pathogens | |||
| Bovine rotavirus (NCDV strain, G6P10 genotype) | CVCC, China | − | − |
| Mycobacterium bovis (MB332, Guangxi field isolate) | CVCC, China | − | − |
| Classical swine fever virus (Guangming stain, AV64, North American genotype) | CVCC, China | − | − |
| Classical swine fever virus (Shimen stain, AV1411, North American genotype) | |||
| Classical swine fever virus (79105 strain, AV63, North American genotype) | |||
| Infective bovine rhinotracheitis virus (Bartha Nu/67 strain, AV20) | CVCC, China | − | − |
| Bovine Coronavirus (GX-BC-125, Guangxi field isolate) | GVRI | − | − |
| Negative tissue samples | |||
| Healthy bovine nasal swab | GVRI | − | − |
| Healthy bovine blood sample | GVRI | − | − |
| Positive tissue sample | |||
| BVDV bovine nasal swab | GVRI | + | − |
| BVDV bovine blood sample | GVRI | + | − |
| Fecal negative samples | |||
| A/Holstein cow/Guangxi/NN1732/2009 | NN | − | − |
| A/Holstein cow/Guangxi/NN3363/2009 | NN | − | − |
| A/Holstein cow/Guangxi/NN4523/2009 | NN | − | − |
| A/Holstein cow/Guangxi/NN4462/2009 | NN | − | − |
| A/Holstein cow/Guangxi/NN21/2009 | NN | − | − |
| A/Holstein cow/Guangxi/NN12/2009 | NN | − | − |
| A/Water baffalo/Guangxi/NN789/2009 | NN | − | − |
| A/Water baffalo/Guangxi/NN35/2009 | NN | − | − |
| A/Water baffalo/Guangxi/NN4520/2009 | NN | − | − |
| A/Water baffalo/Guangxi/NN7/2009 | NN | − | − |
| A/Water baffalo/Guangxi/NN3620/2009 | NN | − | − |
| A/Water baffalo/Guangxi/NN49/2009 | NN | − | − |
| A/Water baffalo/Guangxi/NN332/2009 | NN | − | − |
| A/Water baffalo/Guangxi/NN28/2009 | NN | − | − |
| A/Water baffalo/Guangxi/NN0137/2009 | NN | − | − |
| A/Water baffalo/Guangxi/NN703/2009 | NN | − | − |
| A/Water baffalo/Guangxi/NN46/2009 | NN | − | − |
| A/Yellow cowGuangxi/LZ789/2010 | LZ | − | − |
| A/Yellow cowGuangxi/LZ45/2010 | LZ | − | − |
| A/Yellow cowGuangxi/LZ733/2010 | LZ | − | − |
| A/Yellow cowGuangxi/LZ719/2010 | LZ | − | − |
| A/Yellow cowGuangxi/LZ776/2010 | LZ | − | − |
| A/Yellow cowGuangxi/LZ782/2010 | LZ | − | − |
| A/Yellow cowGuangxi/LZ713/2010 | LZ | − | − |
| A/Yellow cowGuangxi/LZ708/2010 | LZ | − | − |
| A/Yellow cowGuangxi/LZ730/2010 | LZ | − | − |
| A/Yellow cowGuangxi/LZ744/2010 | LZ | − | − |
| A/Yellow cowGuangxi/LZ760/2010 | LZ | − | − |
| Cell cultures | |||
| MDBK 1 | GVRI | − | − |
| MDBK 2 | GVRI | − | − |
| MDBK 3 | GVRI | − | − |
| MDBK 4 | GVRI | − | − |
| MDBK 5 | GVRI | − | − |
CVCC: Chinese Veterinary Culture Collection Center; GVRI: Guangxi Veterinary Research Institute; NN: Jinguang Diary Farm, Nanning, Guanxi Province; LZ: Huangshi Cattle Farm, Liuzhou, Guanxi Province; +: positive; −: negative.