Table 2.
Identification of differentially expressed genes in mouse brain at 1, 2, 4 and 7 days following MHV infection
| Gene/protein name | Fold modulation |
|||
|---|---|---|---|---|
| Day 1 | Day 2 | Day 4 | Day 7 | |
| Immune response related | ||||
| Immune-related | ||||
| Complement component 1 (q subcomponent binding protein) | 2.2 ↓ | 6668.1 ↓ | 1.2 ↓ | – |
| Complement component 1 (q subcomponent alpha polypeptide) | 1.2 ↑ | 1.7 ↑ | 1.2 ↓ | 7.0 ↑ |
| Complement component 1 (q subcomponent beta polypeptide) | 1.4 ↑ | 1.3 ↑ | 1.3 ↑ | 6.7 ↑ |
| Interferon-induced protein with tetracopeptide repeats 3 (IFIT3) | – | 20.6 ↑ | 6.5 ↑ | 16.5 ↑ |
| Interferon dependent positive acting transcription factor 3 gamma (ISGF3 gamma) | 1.3 ↑ | 3.7 ↑ | 3.2 ↑ | 5.1↑ |
| Interferon gamma inducible protein 47 kDa | – | – | – | 30.7 ↑ |
| Interferon activated gene 204 | – | – | – | 5714.1 ↑ |
| Fc receptor, IgE, high affinity 1 (gamma polypeptide) | – | – | – | 19.9 ↑ |
| Fc receptor, IgG, high affinity 1 | – | – | – | 5187.0↑ |
| Fc receptor, IgG, low affinity III | – | – | – | 6.9 ↑ |
| CXCL 9 | – | – | – | 135.5 ↑ |
| CXCL 10 | 65.2 ↑ | 10658.4 ↑ | – | 10813.5 ↑ |
| CD68 Ag | – | – | 1.1 ↑ | 7.7 ↑ |
| CD8 Ag, alpha chain | – | – | – | 8.6 ↑ |
| CD3 antigen gamma polypeptide | – | – | – | 5095.0 ↑ |
| Lymphocyte antigen 86 | 2.1 ↑ | 1.8 ↑ | 2.1 ↑ | 8.8 ↑ |
| High mobility group 1 protein (HMGB1) | 3.7 ↑ | 1.1 ↑ | 1.0 | 17.0 ↑ |
| P lysozyme structural | – | – | – | 4.7 ↑ |
| Cytotoxic granule-associated RNA-Binding protein 1 (TIA) | 1.2 ↓ | 1.4 ↓ | 1.2 ↓ | 5.3 ↑ |
| CCL2/MCP-1 | – | – | – | 5.9 ↑ |
| Antigen processing and presentation | ||||
| Ia-associated invariant chain (Ii) | 1.2 ↑ | 20.6 ↑ | 4.1 ↑ | 123.6 ↑ |
| ATP-binding cassette, sub-family B (MDR/TAP) member 2 | – | – | – | 8166.1 ↑ |
| Cathepsin S | – | – | – | 7.8 ↑ |
| Cystatin F | – | – | – | 25.4 ↑ |
| CD83 Antigen | 3.6 ↑ | 1.2 ↓ | 1.1 ↓ | 7.8 ↑ |
| Extracellular matrix, cell adhesion | ||||
| Galectin-3 | – | – | – | 12.8 ↑ |
| CD18 (integrin beta 2) | – | – | – | 19.3 ↑ |
| Others | ||||
| Transcription | ||||
| Interferon regulatory factor 1 | – | – | – | 13.6 ↑ |
| Heat shock protein, 84 kDa | 20214.2 ↓ | 1.3 ↓ | 1.6 ↓ | 11167 ↑ |
| High mobility group protein 2 | – | – | – | 29.5 ↑ |
| Ribosomal protein S5 | 1.9 ↓ | 1.1 ↓ | 10.2 ↓ | 1.8 ↑ |
| Cellular nucleic acid binding protein | – | 1.8 ↑ | 1.8 ↑ | 4.9 ↑ |
| Signal transduction | ||||
| Protein tyrosine phosphatase receptor type C | – | – | – | 7.3 ↑ |
| PKC isoform iota (PKCI) | 1.1 ↑ | 1.1 ↑ | 5.8 ↓ | 1.1 ↑ |
| Annexin A2 | 1.3 ↓ | – | – | 3.6 ↑ |
| Cell structure, movement and secretion | ||||
| Adaptor protein, complex AP-1, sigma 1 | – | 1.5 ↑ | 16.7 ↓ | – |
| Septin 7/cdc10 | 1.5 ↑ | 3.2 ↑ | 2.0 ↑ | 1.4 ↑ |
| Importin beta | – | 5.4 ↓ | – | – |
| Lysosomal membrane glycoprotein 1 | 1.1 ↓ | 1.1 ↓ | 3.5 ↓ | 1.0 |
| Myelin-Associated glycoprotein (MAG) | 1.2 ↑ | 1.2 ↑ | 3.0 ↓ | 2.2 ↑ |
| GFAP | 1.1 ↓ | 1.2 ↑ | 1.5 ↓ | 4.1 ↑ |
| Vimentin | 1.2 ↑ | 1.2 ↑ | 1.2 ↑ | 4.9 ↑ |
| S100 calcium-binding protein A4 | – | 1.7 ↓ | 2.2 ↑ | 6.8 ↑ |
| Cell division and death | ||||
| Serine protease inhibitor (2–2) | – | – | – | 19.7 ↑ |
| Protein folding | ||||
| FK506 binding protein 6 (65 kDa) | 6951.0 ↓ | – | – | – |
| Chaperonin subunit 6b (zeta) | – | 6137.7 ↓ | – | – |
| Chaperonin subunit 3 (gamma) | 1.5 ↓ | 1.8 ↓ | 3.5 ↓ | – |
| Tubulin cofactor a | – | – | 10.0 ↓ | – |
| Peroxiredoxin 3 | 1.0 | 1.1 ↓ | 1.5 ↓ | 3.2 ↑ |
| Miscellaneous | ||||
| Growth hormone | 7.1 ↓ | 2.6 ↓ | 116.5 ↓ | 9.3 ↓ |
| Prolactin | 6.1 ↓ | 2.5 ↓ | 7.0 ↓ | – |
| Adenylate cyclase activating polypeptide 1 receptor 1 | 1.1 ↓ | 1.3 ↓ | 5680.7 ↓ | – |
| Alcohol dehydrogenase family 3 | – | 5207.7 ↓ | – | – |
| ATP-binding cassette subfamily A (ABC 1) member 1 | – | 5099.0 ↓ | – | – |
| Phosphodiesterase 6D CGMP-specific, rod, delta | – | – | 25.1↓ | – |
| Prosaposin | 1.2 ↓ | 1.8 ↑ | 8.4 ↓ | 1.8 ↑ |
| Lipocalin 2 | – | – | – | 15.3 ↑ |
| Malate dehydrogenase, mitochondrial | 1.2 ↓ | 1.9 ↑ | 2.3 ↓ | 415.0 ↑ |
| Adaptor-related protein complex AP-3 sigma subunit | 1.2 ↓ | 1.2 ↓ | 1.2 ↓ | 9095.1 ↑ |
| Nuclear receptor subfamily 4 group A member 1 | 1.8 ↑ | 1.8 ↑ | – | 5446.9 ↑ |
| LanC/GRP69A/p40 | 1.3 ↓ | 3.1↑ | 1.6 ↓ | 1.6 ↓ |
| Preproacrosin | 1.5 ↑ | 5.1↑ | 5.3 ↑ | 1.5 ↑ |
| Amyloid beta (A4)-like protein 1 | 3.0 ↓ | 1.8 ↓ | 3.4 ↓ | 1.3 ↓ |
| Glutamine fructose-6-phosphate transaminase 1 | – | 6954.9↓ | – | – |
| Mm3 muscarinic acetylcholine Receptor (MACHR) | – | 4.9 ↓ | – | – |
| Glycine receptor beta subunit | 1.1 ↑ | 1.2 ↑ | 5.4 ↑ | – |
| Cytochrome C oxidase Subunit Via, polypeptide 2 | 1.0 | 4.0 ↓ | 1.3 ↑ | 1.1 ↓ |
| Cytochrome b-245, alpha polypeptide | – | – | – | 6.1 ↑ |
| Carbonic anhydrase 11 | 1.8 ↓ | 3.2 ↓ | 1.4 ↓ | 2.0 ↓ |
| Carbonic anhydrase 2 | 1.2 ↓ | 1.6 ↓ | 3.2 ↓ | 1.0 |
| Acetyl-Coenzyme A Dehydrogenase, long chain | – | 3.1 ↓ | – | – |
| Solute carrier family 4 (anion exchanger), member 2 | – | 1.4 ↑ | 3.2 ↓ | – |
| Apolipoprotein CII | – | – | 6.3 ↑ | 2.8 ↑ |
| Apolipoprotein D | 1.7 ↑ | 1.2 ↑ | 1.2 ↓ | 5.7 ↑ |
| Sepiapterin reductase | – | – | 5.7 ↓ | – |
| NADH dehydrogenase Flavoprotein 1 | 1.5 ↓ | 2.0 ↓ | 5.5 ↓ | 1.9 ↑ |
| Glutamate oxaloacetate transaminase 2, mitochondrial | 1.1 ↑ | 1.1 ↑ | 4.3 ↓ | 1.5 ↓ |
| Glucosidase alpha acid | 1.5 ↓ | 1.1 ↓ | 3.5 ↓ | 1.2 ↓ |
| Amyloid beta (A4) precursor-like protein 1 | 3.0 ↓ | 1.8 ↓ | 3.4 ↓ | 1.3 ↓ |
| Heterogeneous nuclear ribonucleoprotein A1 | 1.1 ↓ | 1.5 ↓ | 3.3 ↓ | 2.0 ↑ |
Genes were considered as significantly modulated if the variation between the expression of control and infected mice was at least 3-fold and if the expression level was at least 5000 densitometric units. Values in bold represent significant modulation. The dash symbol (–) means that the densitometric unit was lower than 5000, therefore the modulation could not be addressed according to our criteria.