Table 3.

Proliferation assay associated with the activation of MBP-reactive T cells in spleens of mice at 7 days p.i. with MHV-A59

Mouse number	Positive wells MHV (average cpm)	S.I. (mean)	Positive wells MBP (average cpm)	S.I. (mean)
1	0/24	–	19/26 (3454)	3.1
2	0/25	–	6/28 (3641)	2.6
3	8/26 (5948)	2.2	10/28 (2055)	2.2
4	12/32 (4487)	2.5	7/32 (2596)	2.1
5	6/20 (4226)	2.4	0/24	–
6	0/16		5/23 (3212)	2.3
7	23/26 (5561)	7.1	2/28 (960)	2.1
8	4/24 (1400)	2.3	5/24 (4027)	3.8
9	0/24	–	7/25 (3256)	2.9
10	0/27	–	7/28 (5278)	2.1
11	0/24	–	21/24 (3500)	3.1
12	20/21 (6968)	3.4	1/21 (2265)	2.1
13	2/24 (7835)	2.2	19/24 (8178)	3.6
14	22/24 (7247)	4.5	22/24 (2166)	2.9
15	23/24 (5406)	3.8	19/24 (2883)	3.4
16	10/24 (2677)	2.3	19/24 (3250)	3.0
17	24/24 (7521)	9.1	2/24 (968)	2.1
18	24/24 (2965)	3.4	20/24 (2216)	3.1
19	24/24 (4234)	4.8	14/24 (1568)	2.5
20	21/23 (3086)	3.6	21/23 (2331)	3.3
21	3/24 (753)	2.4	24/24 (1007)	3.5
22	10/15 (1108)	3.1	0/15	–
23	4/24 (2022)	2.3	21/24 (3139)	3.5
24	15/18 (3707)	2.8	18/18 (3571)	3.5
25	19/23 (3204)	2.5	24/24 (3727)	3.6
26	16/16 (3595)	3.4	15/16 (2113)	3.0
27	12/12 (7606)	7.7	1/13 (968)	2.1
28	2/10 (1320)	2.4	0/10	–
29	14/16 (2666)	2.7	16/16 (3294)	3.8
30	3/16 (2198)	2.3	12/24 (2077)	3.6
31	16/16 (8150)	8.6	7/16 (1030)	2.3
Total of wells	339/670		364/702

Thirty mice were infected with 100 PFU and splenocytes were cultivated in 96-well plates as indicated in Section 2.6. The proliferation data (cpm) were considered positive in wells where the count was at least 1000 (before substraction of the background count) and where it was more than twofold superior compared to control antigen (DBT cell lysate for MHV and RPMI culture medium for MBP). Splenocytes from 30 uninfected mice were cultivated the same way. S.I.: Stimulation Index.