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Analytical sensitivity of the TGEV TaqMan assay with fecal samples spiked with different concentrations of the virus. The amplification (A) and the associated standard curve (B) graphs are shown. In panel A, the numbers on each line represent the virus concentration, in TCID50/ml, in the fecal sample. The horizontal line above the X-axis is the threshold line set at 30 fluorescent units. NTC: no template control.
