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. 2009 Sep 1;162(1):231–235. doi: 10.1016/j.jviromet.2009.08.016

Table 1.

Performance of the TGEV TaqMan assay in duplex format with internal amplification control.

Sample Single target assay Ct for TGEV Duplex assay
Ct for TGEV Ct for EGFP
Spiked fecal samples
 107 TCID50/ml 14.29 14.66 0.00a
 106 TCID50/ml 18.75 18.82 27.75
 105 TCID50/ml 22.35 22.74 26.66
 104 TCID50/ml 26.41 26.62 26.51
 103 TCID50/ml 29.68 30.05 27.21
 102 TCID50/ml 34.00 34.07 27.39
 101 TCID50/ml 36.65 37.21 27.42
 100 TCID50/ml 38.84 39.63 26.69
 No template control 0.00 0.00 25.63



Field fecal samples
 1 24.37 24.95 25.58
 2 32.37 32.86 26.65
 3 15.59 15.80 0.00
 4 14.75 14.88 0.00
 5 30.96 31.12 29.30
 6 12.34 12.19 0.00
 7 26.10 26.37 28.60
 No template control 0.00 0.00 25.56
a

No Ct value—no target amplification detected.