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Validation CT12-mCODEHOP primers on total RNA isolated from coronavirus infected cells: two-step RT-PCR assay. The sensitivity of the assay was evaluated on 10-fold dilution series of quantified RNA templates obtained from cells infected with four coronaviruses and one torovirus. Shown are agarose gels electrophoresis with a 1 kb DNA molecular marker, virus-specific amplification bands of ∼200 bp and primer dimer bands.
