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. 2011 Aug 11;177(2):174–183. doi: 10.1016/j.jviromet.2011.08.005

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Copyright © 2011 Elsevier B.V. All rights reserved.

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Fig. 5 — Comparative evaluation of the sensitivity of the CT12-mCODEHOP and CT12-oCODEHOP primer sets toward copy virus RNA templates. The performance of the two primer sets was compared using the one-step RT-PCR assay, and 10-fold dilution series of quantified cRNA templates produced for 5 coronaviruses and one torovirus. Additionally, the effect of the presence of 10¹⁰ copies of cellular RNA (bRNA) per reaction on the performance of the CT12-mCODEHOP primer set was analyzed. Shown are portions of agarose gel electrophoresis images with virus-specific bands.