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. 2006 Oct 9;138(1):218–222. doi: 10.1016/j.jviromet.2006.08.008

Fig. 2.

Fig. 2

Optimisation of BVDV 5′-UTR real-time PCR primers. Mean CT values of (A) serially diluted cDNA from BVDV spiked blood, y = −2.76x + 30.06, r2 = 0.993, (B) plasmid DNA standard curve, y = −3.42x + 35.83, r2 = 0.995, and (C) plasmid DNA (○) and cDNA spiked plasmid DNA (×), y = −3.55x + 38.11, r2 = 0.997. Error bars show standard deviation.