Table 1.

Measurement of cytokine and chemokine mRNA expression levels in tissues by quantitative real-time PCR.

	mRNA	Fold changea				Pd
		Spleen (n = 9)		Lung (n = 9)		Intra-group (lung versus spleen)		Inter-group (EIAVLN40 versus EIAVDLV121)
		EIAVLN40 versus UI	EIAVDLV121 versus UI	EIAVLN40 versus UI	EIAVDLV121 versus UI	EIAVLN40	EIAVDLV121	Lung versus lung	Lung versus spleen	Spleen versus lung	Spleen versus spleen
Cytokines (increased)b	IL-1α	16.9 ± 9.8	4.3 ± 2.2	7.5 ± 2.3	3.5 ± 0.9	NS	NS	NS	NS	NS	NS
	IL-1β	14.8 ± 3.9	2.9 ± 0.6	33.2 ± 17.3	1.0c ± 0.1	NS	0.008	NS	NS	0.003	0.009
	IL-6	269.9 ± 90.5	2.9 ± 0.4	149.4 ± 64.7	2.9 ± 1.2	NS	NS	0.04	0.04	0.009	0.009
	IL-10	25.7 ± 12.8	1.4c ± 0.4	4.2 ± 1.0	1.3c ± 0.3	NS	NS	0.014	0.019	NS	NS
	IFN-α	323.8 ± 228.0	2.6 ± 1.4	9.3 ± 2.9	2.0 ± 1.1	NS	NS	0.009	NS	NS	NS
	IFN-β	28.1 ± 13.2	4.0 ± 2.0	1325.0 ± 872.8	1.5c ± 0.3	NS	NS	NS	NS	NS	NS
	IFN-γ	1.2c ± 0.3	33.4 ± 7.7	14.2 ± 4.0	14.8 ± 4.0	0.002	0.04	NS	0.04	0.004	<0.001
	MX1	5.1 ± 1.0	4.8 ± 1.3	1.4c ± 0.3	3.8 ± 0.9	0.003	NS	0.02	0.019	NS	NS
Chemokines (increased)b	IL-8	535.6 ± 99.0	4.5 ± 1.3	318.7 ± 111.9	4.3 ± 2.2	NS	NS	<0.001	<0.001	0.01	0.01
	CXCL10	147.7 ± 95.2	4.3 ± 0.5	31.8 ± 13.0	3.3 ± 0.5	NS	NS	0.034	0.04	NS	NS
Anti-apoptotic chemokinesb	CXCL12	48.2 ± 37.8	1.4c ± 0.3	2.3 ± 0.8	17.2 ± 2.8	NS	<0.001	0.013	NS	NS	NS
	CCL21	17.0 ± 9.7	1.2c ± 0.6	1.5c ± 0.6	4.7 ± 0.9	NS	NS	NS	NS	NS	NS

UI: uninfected.

NS: not significant.

^aThe arithmetic mean of the fold change of the expression level target gene relative to that of uninfected animals, and data are the mean ± SEM.

^bIncreased expression level of cytokines and chemokines with a fold change >2.

^cUnchanged expression level was defined as fold change <2. Underlined data: decreased expression level with a fold change >2.

^dStudent's t-tests were used for all comparisons.