. 2008 Sep 26;154(1):128–134. doi: 10.1016/j.jviromet.2008.08.007

Table 1.

Comparison of the dynamic range of ISAV segment 8 two tube qRT-PCR with SYBR Green I chemistry and single tube one-step qRT-PCR with TaqMan probe chemistry

	Two tube qRT-PCR with SYBR Green I		Single tube one-step qRT-PCR with TaqMan probe
	NBISA01 (10^8.75 TCID₅₀/ml)	RPC/NB 04-085-1 (10^5.75 TCID₅₀/ml)	NBISA01 (10^8.75 TCID₅₀/ml)	RPC/NB 04-085-1 (10^5.75 TCID₅₀/ml)
Dynamic range (TCID₅₀/ml)	10^0.75	10^0.75	10^1.75	10^1.75
Reliable detection^a limit (TCID₅₀/ml)	10^4.75	10^2.75	10^4.75	10^2.75
Reliable detection^a limit (ISAV RNA copy number equivalents/ml)	5956.4 ± 24.3	18568.1 ± 539.1	27.7 ± 1.8	227.2 ± 55.8

Reliable detection is defined as the dilutions run in triplicate giving similar Ct values.