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. 2009 Feb 4;158(1):41–45. doi: 10.1016/j.jviromet.2009.01.014

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Copyright © 2009 Elsevier B.V. All rights reserved.

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Fig. 2 — Standard curves generated using 10 fold dilutions of template RNA prepared from the Lelystad (A) and Ingelvac MLV^® (B) strain. (Three replicates were performed of each dilution, average values, and standard deviations are shown in the curves.) The RNA copy number is indicated on the x-axis, the C_t values on the y-axis. The R² (correlation effiecient) was 0.99 in both cases, whereas the E (efficiency) was 0.99 in case of the Lelystad virus and 1.00 in case of the the Ingelvac MLV^® strain.