Fig. 1.

The schematic of the universal microarray-mediated by LDR. (A) The genotype of every mutation base can be detected by a set of oligonucleotides including a discriminating oligonucleotide and a labeled oligonucleotide. The discriminating oligonucleotide contains a cZip Code at 5′ end and a hydroxy group at 3′ end. The labeled oligonucleotide was phosphorylated at 5′ end and is affixed a Cy3 to 3′ end. The two sequences at the junction must be matched. (B) The spotting sequences consist of a Zip Code at the 5′ end and the (dA)₁₅-NH_{2 H2h}at 3′ end; the discriminating oligonucleotide includes cZip Code at the 5′ end and the hybridization sequence at 3′ end.