Table 3.
The discriminating oligonucleotides and the labeled oligonucleotides
| Sites | Discriminating oligonucleotide (5′ → 3′) | Labeled oligonucleotide (5′ → 3′) |
|---|---|---|
| 1 | TGGCGAGAGTGTCTCGTCGATCATCCTACTACTTTATGAAATTCAGACGTGC | p-TTTTGGTGAGTACAACCATGTTGTT-Cy3 |
| ATCTTGCGCGGCAGCTCGTCGACCGCTACTACTTTATGAAATTCAGACGTGT | ||
| AAAGCGGGCGGCGATCGCGAATGTCCTACTACTTTATGAAATTCAGACGTGA | ||
| 2 | AGATTGGGATGCGGTCGCGATACCGGATGTCTTTCACTATACTCTGTCTGGT | p-ACCAGCTTACAGCTTTCTGCCGG-Cy3 |
| GAGGATCTGTAGCGCCTCTTCGAGCGATGTCTTTCACTATACTCTGTCTGGC | ||
| CGAACTCGAAGCCGAGCTGGCGGTGTTTGATGTCTTTCACTATACTCTGTA | ||
| 3 | GATCGGCCGGTGAAGCGAAAGGTTCAAAAGAGAAGCCCCAGCACATGTA | p-TCTACAATAGGTGTCTGCACAATGACT-Cy3 |
| GATGGTGATCCCGCGCGTGCCGAAAAAAAGAGAAGCCCCAGCACATGTG | ||
| GGATTGCACCGTCAGCACCACCGAGAAAAGAGAAGCCCCAGCACATGTT | ||
| 4 | TCCCAGGACGGCGCTGGCACGTTGAGTTTCATACTATTAATCATACGTTTGG | p-CAACCCTGTCATACCTTTTAAGGA-Cy3 |
| CGGCGTCCACGTCGAGTTCCTTCGCGTTTCATACTATTAATCATACGTTTGA | ||
| TGTGCGCCCGAGATCGGTATCCCCGGTTTCATACTATTAATCATACGTTTGT | ||
| 5 | ATCGCATCGTGATGGCGTAAGCTCCAGCCTTTTCACCTGCTCAAGACAT | p-TTGGGGCACGTCAGCTGCAGC-Cy3 |
| TTCGGGGAAACTCCGCACCGCCACGAGCCTTTTCACCTGCTCAAGACAC | ||
| TAGGTTTGGCCAGTGCGTTGGATCGAGCCTTTTCACCTGCTCAAGACAA | ||
| 6 | TCGACAACCCGGTTGGAGGATTCAGTTGTATTTCTCTATGCAGTTGCATAT | p-GCACTGTAGTACAGCGCTGTGC-Cy3 |
| CCAAAAGCTTTACGCCAGCGCCGAATTGTATTTCTCTATGCAGTTGCATAC | ||
| CCGTACCCTTCCGCTGGAGATTTACTTGTATTTCTCTATGCAGTTGCATAAA | ||
| 7 | The hybridization control cZip Code | GGGTATCCGTTCGGTGTTGCGTAGT-Cy3 |
The italic sequences are the cZip Codes and the bold and italic bases are the mutated bases. Tm value of the discriminating oligonucleotides is about 65 °C and Tm value of the labeled oligonucleotides is about 55 °C.