Table 2.

NQPCR quantification result of 10-fold serially diluted BCoV RNA into the stochastic zone (1–10 copies per PCR reaction).

	Estimated copy number per PCR reaction
	100	10	1a	0
Dilution 1	107	0	0	0
Dilution 2	95	10	0	0
Dilution 3	119	0	0	0
Dilution 4	67	16	0	0
Dilution 5	63	0	0	0
Dilution 6	109	0	13	0
S.D.b	23.27	6.98	5.31	0

Six different tubes were analysed per dilution. The estimated RNA concentration in copies per PCR reaction, in relation to the Threshold cycle (C_t) is shown.

^aAt the estimated concentration of one copy per PCR reaction one of six samples were positive.

^bStandard deviation.