Table 3.
Results obtained for the positive group with conventional and molecular methods
| Viruses | No. of specimens tested | DFA | No. of positive on MRC5 | No of positive on HuH7 | hMPV RT-PCR positive | No. of specimens positive with RT-PCR multiplex 1 | No. of specimens positive with hemi-nested PCR multiplex 1 | No. of specimens positive with RT-PCR multiplex 2 | No. of specimens positive with hemi-nested PCR multiplex 2 | No. of specimens positive with RT-PCR multiplex 3 | No. of specimens positive with specific hemi-nested PCR |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Influenza A virus | 4 | + | − | 4 | 4 | − | − | − | |||
| 5 | ++ | − | 5 | 5 | − | − | − | ||||
| 3 | +++ | − | 3 | 3 | − | − | − | ||||
| 8 | − | − | 8 | 8 | 8 | − | − | − | |||
| Influenza B virus | 5 | + | − | 5 | 5 | − | − | 1hRV | 1hRV | ||
| 2 | ++ | − | 2 | 2 | − | − | − | ||||
| 3 | − | − | 3 | 3 | 3 | − | − | − | |||
| hMPV | 20 | − | − | − | 20 | 20 | 20 | − | 2hRV | 2hRV | |
| hRSV | 5 | + | 5 | 5 + 1hMPV | 5 + 1hMPV | − | − | − | |||
| 7 | ++ | 7 | 6 + 2hMPV | 7 + 2hMPV | − | − | 1hRV | 1hRV | |||
| 2 | +++ | 2 | 2 | 2 | − | − | − | ||||
| 3 | + | − | 3 | 3 | − | − | − | ||||
| 2 | ++ | − | 2 | 2 | − | − | − | ||||
| 4 | +++ | − | 4 + 1hMPV | 4 + 1hMPV | − | − | 1hRV | 1hRV | |||
| 7 | − | 7 | 1 | 7 | − | − | 5hRV | 5hRV | |||
| PIV-1 | 1 | − | − | 1 | − | − | 1 | 1 | − | ||
| PIV-2 | 3 | − | − | 3 | 1hRSV | 1hRSV | 1 | 3 | 1hRV | 1hRV | |
| PIV−3 | 5 | + | − | 1hMPV | 1hMPV | 5 | 5 | 2hRV + 1OC43 | 2hRV + 1OC43 | ||
| 2 | ++ | − | − | − | 2 | 2 | 1hRV | 1hRV | |||
| 2 | +++ | − | − | − | 2 | 2 | − | ||||
| 1 | − | − | 1 | − | − | 1 | 1 | 1hRV | 1hRV | ||
| 8 | − | 8 | 2hMPV | 2hMPV | − | − | 8 | 8 | |||
| hRV | 5 | − | − | 5 | 1hMPV | 1hMPV | − | − | 5 | 5 | |
| 1 | − | 1 | 1hMPV | 1hMPV | − | − | − | − | |||
| 1 | − | 1 | − | − | 1 PIV-4 | 1 PIV-4 | − | − | |||
| HCoV OC43 | 2 | − | − | 2 | − | − | − | − | 2 | 2 | |
DFA+: low positive intensity; DFA++:medium positive intensity; DFA+++: strong positive intensity; (+): positive result; (−): negative result. Bold: detected only with multiplex method.