Table 2.
ASFV recombinant antigens used for the swine immunogenicity study.
| Gene Feature | Gene Name |
||||
|---|---|---|---|---|---|
| E183L | EP402R | EP153R | O61R | B646L | |
| Vaxign Ranking | 4 | 15 | 17 | 20 | 107 |
| Protein ID | CBW46791.1 | CBW46724.1 | CBW46723.1 | CBW46764.1 | CBW46748.1 |
| Length (aa) | 184 | 360 | 158 | 61 | 646 |
| Predicted size (kDa) | 19.9 | 41.0 | 18.4 | 6.7 | 73.2 |
| Adhesin Probability score | 0.566 | 0.457 | 0.372 | 0.41 | 0.248 |
| Transmembrane helices | 1 | 1 | 1 | 1 | 0 |
| hMHC-I epitopes score | 0.668 | 0.685 | 0.730 | 0.636 | 0.653 |
| hMHC-II epitopes score | 0.729 | 0.659 | 0.781 | 0.632 | 0.739 |
| Present in all 12 ASFV genomes (Y/N) | Y | Y | Y | Y | Y |
| Protein localization on viral particle | Viral capsid | Viral capsid | Localized to host cell | Viral capsid | Viral capsid |
| Gene function | Virus entry (Gómez-Puertas et al., 1998) | RBC hemadsorption (Borca et al., 1994b) | Enhances RBC hemadsorption (Galindo et al., 2000) | Viral attachment protein (Gómez-Puertas et al., 1998) | Viral capsid protein (Borca et al., 1994a) |
The Georgia 2007/1 strain genome was used as the seed genome. Vaxign ranked each protein based on predictions for the following features: the number of transmembrane helices, adhesin likelihood score, MHC-I and II epitopes score, and sequence conservation among 12 ASFV strains. The range of the feature score unit is 0–1; 0 is minimum and 1 is maximum. The range of the Vaxign rank is 1–192; 1 is maximum and 192 is minimum; 192 corresponds to the total number of identified ORFs within the ASFV genome.