Table 1.
A summary of the nucleic acid extraction systems used in this study.
| Instrument/Kit | Protocol/chemistry | Core technology | Maximum sample volume (μL) | Length of automated protocol (min) | Cost per extractiona | Maximal extractions per run | |
|---|---|---|---|---|---|---|---|
| 1 | Allprep | Manuala | Glass fiber filter | 100b | N/A | $7.28 | N/A |
| 2 | Biorobot MDX | Robotic/One-For-All NA kit | Glass fiber filter | 263 | ∼150 | $3.36 | 96 |
| 3 | KingFisher mL | Robotic/IviMag Bateria Kit | Magnetic beads | 400 | 20 | $3.51 | 15 |
| 4 | Biorobot EZ1 | Robotic/DNA Tissue Kit | Magnetic beads | 200 | 20 | $6.85 | 6 |
| 5 | easyMAG | Robotic | Magnetic beads | 1000 | 45 | $5.63c | 24 |
| 6 | KingFisher Flex | Robotic/IviMag Bateria Kit | Magnetic beads | 400 | 20 | $3.83d | 96 |
| 7 | MagNA Pure Compact | Robotic/NA Isolation Kit I | Magnetic beads | 400 | 20 | $7.68 | 8 |
a
The cost per extraction was calculated by dividing the cost of each kit, based on pricing quotes from each manufacture in early 2010, by the number of samples that could be extracted by each kit.
b
For efficient sample lysis, the sample volume should not exceed 100 μL, although the maximum loading volume is 700 μL.
c
The cost per extraction was quoted for off-instrument lysis.
d
The cost per extraction calculation included the cost of 96-well plates (AppliedBiosystems, Cat# 4388476) and combs (AppliedBiosystems, Cat# 4388487) that are sold separately.