Table 1.

Summary of isothermal nucleic acid amplification techniques.

Isothermal technique	Target	Primers needed	Initial heating	Incubation temperature (ºC)	Amplification time (min)	Limit of detection (copies)	Multiplexing	Lyophilised reagents	FDA approved tests
NASBA	RNA	2	No	41	60–180	1	Yes	Yes	Yes
SDA	DNA	4	Yes	30–55	60–120	10	Yes	No	Yes
RCA	DNA/RNA	1	Yes	30–65	60–240	10	No	No	No
LAMP	DNA	4–6	Yes	60–65	60	≈5	Yes	No	Yes
HDA	DNA	2	No	65	30–120	1	Yes	No	Yes
RPA	DNA/RNA	2	No	37–42	20–40	1	Yes	Yes	No