Table 2.

RPA methods developed for lateral flow strip detection.

Organism	Target	Sample	Heat source	Amplification time (min)	Temperature (T) (ºC)	Limit of defection (LOD)	Ref.
Methicillin resistant Staphylococcus Aureus (MRSA)	dsDNA	Genomic DNA	Not specified	<30	37	10 copies	[4]
HIV	dsDNA	Plasmid template	Heat block	15	37	10 copies	[64]
Cryptosporidium	dsDNA	Human stool spiked with cryptosporidium	Heat block	30	37	1–10 copies	[65]
HIV-1	dsDNA	Plasmid template with human genomic DNA background	Body heat	20–30	Body heat (≈31–35)	10 copies	[11]
Plasmodium falciparum	dsDNA	Genomic DNA	Incubator	10	38	10 fg	[8]
Chlamydia trachomatis	dsDNA	Urine	Incubator	10	38	50 copies	[47]
HIV-1	dsDNA	Infected cell line	Incubator	20	39	1–3	[61]
HIV-1	dsDNA	Infected cell line	Chemical heater	20	10–44	10	[9]
Orientia tsutsugamushi	dsDNA	Blood from infected patients and infected mice	Heat block	20	39	53 copies	[68]
Rickettsia typhi	dsDNA	Human plasma spiked with R. typhy cells	Heat block	20	39	20 copies	[68]
Plasmodium	dsDNA	Plasmid template	Hot plate	30	37	50	[66]
aGiardia, cryptosporidium and Entamoeba	dsDNA	Live parasites spiked into stool	Heat block	35	37	≈400 copies (triplex)	[57]
HIV-1	dsDNA	Plasmid template	Heat block	30	37	1000	[45]
HIV-1	dsDNA	Infected cell lines	Portable heat block	20	39	10	[12]
Giarda duodenalis	dsDNA	Stool	Incubator	30	37	10	[69]
Pork breed mangalica	dsDNA	Meat, sausages and paté	Twirla	30	39	1 copy	[70]
Listeria monocytogenes	dsDNA	Genomic DNA spiked on pork, chicken, beef, fish and milk	Dry bath	20	37	1360 CFU/mL	[71]
Borrelia burgforferi	dsDNA	Cell lines and serum samples	Thermoshaker	20	37	25 copies	[72]
Leishmania Viannia spp	dsDNA	Skin ulcera	Dry bath	30	45	0,1 parasite	[73]
Schistosoma japonicum	dsDNA	Stool samples	Twista	20	39	5 fg	[74]
Cryptosporidium	dsDNA	Feces	Thermoshaker	25	37	0,5 oocyst	[50]
Caprine arthritis-encephalitis virus	dsDNA	Blood	Not specified	30	37	10 copies	[75]
GM soybean	dsDNA	Seeds	Incubator	20	39	10 copies	[10]
Orf virus	dsDNA	Nasal swabs, skin, lymphatic nodes, liver, lungs, stomach and kidneys	Water bath	20	37	80 copies	[76]
bSynthetic target	dsDNA	Artificial sample	Incubator	15	37	10−11 M	[67]
Penaeus stylirostris virus	ssDNA	Muscles of shrimps	Heat block	30	35–40	100 copies	[77], [78]
bΒ-conglutin	ssDNA, aptamer	Artificial sample	Room temperature	15	Room temperature	0,17 amol	[79]
cYellow fever virus	ssRNA	Cell culture supernatant and mosquito pools	Heat block	20	39	<21	[80]
cPeste des petits rumiants virus	ssRNA	Tissues	Thermocycler	20	39	150 copies	[81]
dLittle cherry virus 2	ssRNA	Leaves, budwood, mealybugs and cherry tissue	Incubator	15	39	–	[82]
d Plum pox virus	ssRNA	Peach, apricot, plum, cherry tree leaves	Portable heat block	15	39	1.0 fg	[83]
dTomato chlorotic dwarf viroid	ssRNA	Leaves, potato, petunia plant, seeds.	Incubator	15	39	100 fg-1pg	[84]

^aTriplexing

^bTwistAmp Basic kit. Use of tailed primers instead of antigen-primers and antibodies/streptavidin.

^cTwistAmp RT nfo.

^dAmplifyRP^R and Acceler8™