Table 4.3.

Real-time RPA methods based on fluorescence detection using alternative devices.

Organism	Target	Sample	Kit/extra reagents	Amplification device, Detection platform	Amp. time (min)	T (ºC)	LOD	Ref.
Klebsiella pneumoniae	dsDNA	Urine	TwistAmp Exo kit	Microplate reader	20	39	1000 UFC/ml	[21]
Chlamidia trachomatis	dsDNA	Synthetic DNA	TwistAmp Fpg kit	Homemade heating block and optical system	40	44	100,000 copies	[130]
MRSA	dsDNA	PCR amplicon	TwistAmp Exo kit	Microfluidic lab on a foil, Real-time rotatory analyser	<20	37	<10 copies	[58]
Group B Streptococci and B. atrophaeus	dsDNA	Vaginal and anal	TwistAmp Exo kit	Real-time rotatory analyser	<20	39	20 copies	[131]
Clostridium difficile	dsDNA	Cell cultures	TwistAmp Exo kit	Slip-chip, Real time thermocycler	<20	39	1000 copies	[96]
Antibiotic resistance Escherichia coli	dsDNA	Cell culture	TwistAmp Exo kit	Digital microfluidic on an AM-EWOD device	15	39	1 copy	[13]
Salmonella enterica, Escherichia coli O157:H7, vibrio parahaemolyticus	dsDNA	Milk	TwistAmp Exo kit	Centrifugal microdevice, Custom made portable genetic analyser with a miniaturized optical detector	20	39	4 cells	[49]
Zika Virus	RNA	Urine	TwistAmp RT Exo kit	Modified 3D printer, Blue laser, mobile phone camera and filter	12	40	5 PFU/ml	[97]