Table 5.

Alternative real-time detection approaches.

Organism	Target	Sample	Kit/extra reagents	Amplification device	Transduction	Detection platform	Amp. time (min)	T (ºC)	LOD	Ref.
Mycobacterium tuberculosis	dsDNA	Sputum	TwistAmp Basic kit	Silicon microring resonator	Wavelength shift	IR sensor	20	37	26 pg/mm2	[29]
Francisella tularensis	dsDNA	DNA template	TwistAmp Basic kit	Silicon microring resonator	Wavelength shift	IR sensor	60	37	600,000 copies/uL	[28]
Human cancer cells	dsDNA	Bladder cancer cells	TwistAmp Basic kit	Silicon microring resonator	Wavelength shift	IR sensor	20–30	37	500fg/uL	[27]
Plasmodium falciparum	dsDNA	Whole blood	TwistAmp Basic kit	Sensor chip + heating plate	Phase change	Mach-Zehnder interferometer	30	37	1 parasite/uL	[132]