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. 2017 Oct 26;98:19–35. doi: 10.1016/j.trac.2017.10.015

Table 6.

Absolute quantification strategies.

Organism Target Sample Kit/extra reagents Amplification device Transduction Detection Platform Amp. time (min) T (ºC) LOD Ref.
MRSA dsDNA Genomic DNA TwistAmp Exo kit SlipChip + plate reader Fluorescence Plate reader 30 39 300 copies/ml [134]
MRSA dsDNA Genomic DNA spiked in blood TwistAmp Exo kit Microfluidic chip, digital plasma separation, incubator Fluorescence Fluorescence microscope 30 37 1000 copies/ml [20]
Listeria monocytogenes dsDNA Certified DNA TwistAmp Nfo kit Centrifugal heater with an integrated fluorimeter Fluorescence Centrifugal heater with an integrated fluorimeter 30 39 – [133]
Listeria monocytogenes dsDNA Genomic DNA TwistAmp Exo kit Picoliter array chip Fluorescence Modified fluorescence microscope 15 39 4·10−3 copies/well [135]