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Effect of heat treatment on the infectivity of SARS-CoV. Virus aliquots (400 μl) were incubated at (A, C) 56 °C, (B, C) 65 °C and (C) 75 °C. Samples were removed at the designated time, frozen, and titrated in Vero E6 cells in triplicate. The dotted line denotes the limit of detection of the assay.
