Table 2.
Primers used for RT-PCR detection and sequencing the whole H gene.
| Oligonucleotides | Nucleotide locationa | Sequence | Amplicon |
|---|---|---|---|
| P1 | 198 | 5′GATAAAGCATGTCATTATAGTCCTAA3′ | 335bp |
| P2 | 533 | 5′CTTGAGCTTTCGACCCTTC3′ | |
| P3 | 8046 | 5′GGGCAACACCTATGGATCAAG3′ | 555bp |
| P4 | 8601 | 5‘TGGACTCAGTAAGGACATCTT3′ | |
| HC-1 | 7079 | 5′AACTTAGGGCTCAGGTAGTC3′ | 2023bp |
| HC-2 | 8902 | 5′AGATGGACCTCAGGGTATAG3′ |
Location of nucleotides was based on the sequence of the complete gene of the CDV, Onderstepoort strain.